http://localhost:8080/handle/prefix/91 2026-11-11T10:11:32Z 2026-11-11T10:11:32Z Silva, Rosinildo Galdino http://localhost:8080/handle/prefix/165 2019-06-24T13:44:14Z 2018-01-01T00:00:00Z

Title: Repositor isotônico de açaí: caracterização físico-química, atividade antioxidante e vida de prateleira Autor(es): Silva, Rosinildo Galdino Primeiro Orientador: Neves, Leandro Timoni Buchdid Camargo Abstract: The biodiversity of the Amazon region provides studies in the most diverse areas of knowledge. Exploring this diversity in the quest for a better quality of life or in improving clinical conditions or for physical performance issues becomes increasingly common, including for the purpose of producing regional fruit products that bring some benefit to the consumer. The objective of this research was to elaborate a hydroelectrolytic repository of açaí enriched with vitamins and creatine and to evaluate the physicochemical characteristics, the shelf life and the antioxidant activity of the repository at time 0, 7, 14, 21 and 28 days after its preparation. After the initial analyzes in the pulps (centesimal composition, physicochemical characteristics), four formulations of the repository containing 5, 10, 15 and 20% of pulp in their initial volume and added with vitamins and creatine (CS5, CS10, CS15 and CS20) and control formulations (SS5, SS10, SS15 and SS20) were developed for comparison purposes. CS drinks were evaluated to determine if they were within the current legislation, and SS and CS were subsequently subjected to determinations of physical chemical variables (pH, titratable acidity, soluble solids, SS/AT ratio) as well as for the determination of bioactive compounds (total phenolics and total anthocyanins) and antioxidant activity by the DPH method. The microbiological evaluation was determined by coliform analyzes at 45ºC and presence of Salmonella sp. The CS formulation drinks were also submitted to sensory analysis (preference-order and hedonic scale test) and both were evaluated for shelf life. The results showed that the CS formulations had their sodium and potassium concentrations within the parameters required for hydroelectrolytic repositories. However, its osmolality has been shown to be slightly elevated without reaching levels that may adversely affect the body's water balance or cause gastrointestinal discomfort. The physical-chemical characteristics in SS and CS showed variations within the storage period without being very different from the values observed in the main commercial beverages evaluated in other studies. The total phenolics and anthocyanins contents of the CS formulations showed high values at the end of the evaluated period, as well as the antioxidant activity, being able to be an ally in the fight against the harmful effects of free radicals in the body cells. Regarding the microbiological evaluation, there were no significant alterations of the analyzes used as parameter in this study, indicating its stability. The sensorial tests indicated for most attributes values higher than the cut grade (5) and CS15 and CS20 were significantly different to CS5. Based on the results, it is concluded that the beverage proposed here, although slightly hypertonic, partially complies with the legislation for hydroelectrolytic repositories and the addition of vitamins and creatine fulfills its purpose of raising total phenolic content and antioxidant activity, be a source of compounds with anti-free radical action. Publisher: Universidade Federal de Roraima Tipo: Tese

2018-01-01T00:00:00Z Duarte, Ednalva Dantas Rodrigues da Silva http://localhost:8080/handle/prefix/164 2019-06-20T05:00:12Z 2018-01-01T00:00:00Z

Title: Caracterização química e da matéria orgânica de solo em ambientes de veredas e lagos nas savanas do norte amazônico Autor(es): Duarte, Ednalva Dantas Rodrigues da Silva Primeiro Orientador: Melo, Valdinar Ferreira Abstract: The environments of buriti lakes and trails (Mauritia flexuosa) are of great ecological importance for the environmental sustainability of the Roraima savanna. The objective in this work was to study soil organic matter (SOM) of lakes and buriti riparians of the Roraima savanna, chemical attributtes (pH, P, K+, Mg2+, Ca2+, Al3+, Al + H, C, and N), cation exchange capacity (CEC), sum of base, base saturation (V) and enzymatic activity of urease and cellulase. The study was carried in the municipality of Boa Vista, Roraima. The soils were collected in five reparians (Embrapa, Casai, Operário, Bom Intento and Bosco) and three lakes (Manaíra, CCA and Embrapa). The soil samples were submitted to chemical and biochemical analyzes, besides the extraction and fractionation of the humic substances. As soil samples were also characterized by CHN and 13C-NMR. The results were as follows: Histosols and Gleysols, strongly acidic, most environments presented pH range from 4.1 to 4.5, low available Ca2+, Mg2+, K+ and P contents, demonstrating the low fertility. The P available in the Bom Intento riparian was high and can be attributed due to the anthropic action. The values obtained for the sum of base were low, and for CEC it was high. The Al3+ contents were high and characterize the soils as allitic. The enzymatic activity presented variation between the environments lakes and buriti riparians, demonstrating that they are sensitive to anthropic action. The 13C-NMR spectra revealed that the aromatic organic compounds are predominant in the studied soils. Humin fraction (C-HUM) is predominant as fractions of the humic substances of these soils. According to the results, the soils of lakes and buriti trails of the savanna of Roraima can function as a source of C and are sensitive to anthropic action and require attention of policy actions aimed at the conservation of these areas. Publisher: Universidade Federal de Roraima Tipo: Tese

2018-01-01T00:00:00Z Santos, Ricardo Carvalho dos http://localhost:8080/handle/prefix/163 2019-06-20T05:00:13Z 2016-06-27T00:00:00Z

Title: Bioprospecção de ácidos graxos, α-TOCOFEROL atividade Anti- Acetilcolinesterásica em óleos e gorduras de frutos amazônicos Autor(es): Santos, Ricardo Carvalho dos Primeiro Orientador: Chagas, Edvan Alves Abstract: The plants species Açaí (Euterpe oleracea), ata brava (Annona hypoglauca), bacaba (Oenocarpus bacaba), buriti (Mauritia flexuosa), buritirana (Mauritia aculeata), murici amarelo (Byrsonima crassifolia), murici vermelho (B. coccolobifolia), piaçava brava (Barcella odora), pupunha yello and red varieties (Bactris gasipaes) and tucumanzinho (Astrocaryum acaule) are common plants in the flora of the Roraima state, some plants above do not provide information or they are incipient as the profile of saturated and unsaturated fatty acids (SUFA), α-tocopherol (vitamin E) and activity on acetylcholinesterase (AChE) inhibition. For this reason, the objective was to conduct a bioprospecting of crude oils and fats pulp and seeds from the Amazon against the SUFA profile, α- tocopherol and anti-acetylcholinesterase activity of the above species. SUFA was analyzed by a Gas Chromatograph coupled to Flame Ionization Detector (GCFID), Hydrogen Nuclear Magnetic Resonance (1H NMR) and Infrared (IV). α- tocopherol was analyzed by High-Performance Liquid Chromatography (HPLC). Since anti-acetylcholinesterase activity was evaluated in vitro in an ELISA plate reader. The results point to a high concentration of oleic acid in most oils and fats, followed by linoleic and linolenic acids. There is the presence of vitamin E in all oils except: tucumanzinho and pupunha yellow and red varieties. The inhibition activity of the enzyme AChE was observed in all samples. Publisher: Universidade Federal de Roraima Tipo: Tese

2016-06-27T00:00:00Z Brandão, Márcia Brazão e Silva http://localhost:8080/handle/prefix/161 2019-06-25T05:00:08Z 2018-01-01T00:00:00Z

Title: Diversidade de cepas produtoras de betalactamases de espectro estendido em um hospital público de Boa Vista-RR Autor(es): Brandão, Márcia Brazão e Silva Primeiro Orientador: Granja, Fabiana Abstract: The emergence of antimicrobial resistant bacteria is occurring all over the world at a faster rate than the discovery of new drugs, putting their registration at risk. Bacteria of the extended-spectrum beta-lactam-producing Enterobacteriaceae (ESBL) are widely disseminated in hospital settings. The incorrect disposal of clinical waste in hospital effluents, allowing the antimicrobial resistance clinics to be more recent and demonstrate environmental risk insofar as they allow the dissemination of this resistance. This research was presented with a series of information that characterized the antimicrobial agents in clinical strains and a picture with the presence of hospital nurses, the faces followed, the dissemination of pathogenic microorganisms in the environment. The aim of this research is to characterize a diversity of hospital strains of the General Hospital of Roraima (HGR) comparing them with health clinics without the same hospital and to conduct a survey of resistance genes of the families: TEM, SHV and CTX, responsible for ESBL coding and hydrolysis to antimicrobials of the class of cephalosporins, penicillins and monobactans. For the proposed proposal, we included the clinical data of patients hospitalized in the HGR and collected hospital septic tank samples in a period of June 2014 in June 2016 in a molecular study to identify resistance genes through the chain reaction polymerase chain reaction (PCR) and sequencing test by the Sanger method. The results showed greater clinical relevance regarding the detection of ESBL, were of urine samples, catheter tip, tracheal secretion and blood. The resistance profile in samples from patients and children is identified as piperacillin / tazobactam, imipenem and amikacin as the most sensitive drugs for ESBL-producing bacteria, as well as the predominance of Klebsiella sp. Among the problems of the TEM gene among the coding of beta-lactamases. ESBL genes were identified in frequencies and isolated and in combination. A characterization of the ESBL subtypes evidences a presence of the CTX-M-15 gene, more frequent worldwide, besides the presence of the SHV gene and the TEM-181 gene in Effluent strains. The results indicate a wide dissemination of the ESBL genes in the clinical strains and in the effluent of HGR. Publisher: Universidade Federal de Roraima Tipo: Tese

2018-01-01T00:00:00Z